<- Home <- Arhive <- Vol. 17, Issue 1, March 2009

Rom J Leg Med17(1)51-58(2009)
© Romanian Society of Legal Medicine

Automated DNA isolation for databasing purposes

F. Stanciu, I.M. Stoian, O.R. Popescu

Abstract: Nowadays, automation is crucial in databasing laboratories especially for countries which are relative new in the field and must quickly reach the minimum standards of consecrated databasing laboratories from Europe and USA. In our efforts to supply genetic profiles for Romanian National DNA Database, we evaluated the Freedom Evo 150 liquid handling platform with AGOWA sep9600 magnetic separator in different running conditions and workflows, for reaching the most efficient ones. We compared the results with those obtained from manual DNA isolation with Chelex resin and paramagnetic particles. For this we used a total of 6246 biological reference samples (5220 - automated processed and 1026 - manually processed), amplified using AmpFlstr Identifiler Kit and analyzed by capillary electrophoresis using ABI Prism 3100 Genetic Analyzer.
Keywords: automation, databasing laboratory, liquid handling platform, DNA magnetic separator.

Full Text in PDF
© 2008-2020 Romanian Society of Legal Medicine. All rights reserved
created by cooz.ro