Haplotype analysis of 8 Y-STR loci in the Antalya population

D. Sema, T. Serdar

Abstract: The haploid and non-recombining nature of most Y-chromosomal short tandem repeats (Y-STRs) and their relative simplicity make them useful tools for male identification purposes in forensic investigations. STR polymorphisms on the non-recombining part of the Y-chromosome are inherited in the form of compound Y-haplotypes and it is known that they are strongly population specific. To contribute to regional database, we have analysed the Ychromosome specific STR (Y-STR) loci including DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393 and DYS 385 in a group of Turkish males from Antalya, Turkish Republic. Unrelated healthy 210 males who have been living in this region for at least three generations and who were involved in legal proceedings concerning paternity or identity were included in the study. PCR analysis was performed on genomic DNA obtained from peripheral blood samples. PowerPlex Y kit was used according to the manufacturer’s recommendations for the amplification and typing procedures. Capillary electrophoresis was carried out on an ABI Prism 310 Genetic Analyzer. We determined a total of 177 haplotypes and 151 of them were observed only once. Twenty six haplotypes identified in more than one male, 21 of them were shared by two different individuals, three by 3 individuals and 2 by four individuals. The most common haplotypes, “14/12/28/23/10/11/12/13- 18” and “15/13/29/24/10/11/14/13- 14” repeated four times, with a frequency of 1.9%. The determined haplotypes of this study were compared with the datas in the Y-chromosomal STR Haplotype Reference Database (YHRD) and also with some available studies from pubmed results for Turks.
Keywords: Y chromosome, Y-STR, polymorphism, haplotype, Turkish population, forensic